Protein engineering is one of the most powerful and remarkable developments in biotechnology; has been used over decades to manipulate amino acid sequence at gene level leading to novel protein with desired functions. Construction of gene at will in order to produce new protein structure is no longer dream due to the advancements in the field of genetic engineering. This exciting area of research has enormous commercial applications with huge impact on industry ranging from drug designing to stem cell research, bio engineering, chemical industry, environment, food and agriculture. Protein engineering is relatively new field gaining lots of attention, has had major breakthrough in medical science with the production of insulin; may play vital role in nearly every field in future. The primary aim of my talk is to give students and other audience an overview of genetic modification and their applications in biotechnology. This talk essentially provides a basic understanding and insight in the prevailing approaches and strategies used in protein engineering in order to generate efficient and novel protein with improved functionality.